Proximity-dependent labeling identifies dendritic cells that drive the tumor-specific CD4⁺ T cell response.

ABSTRACT: Dendritic cells (DCs) are uniquely capable of transporting tumor antigens to tumor-draining lymph nodes (tdLNs) and interact with effector T cells in the tumor microenvironment (TME) itself, mediating both natural antitumor immunity and the response to checkpoint blockade immunotherapy. Using LIPSTIC (Labeling Immune Partnerships by SorTagging Intercellular Contacts)-based single-cell transcriptomics, we identified individual DCs capable of presenting antigen to CD4⁺ T cells in both the tdLN and TME. Our findings revealed that DCs with similar hyperactivated transcriptional phenotypes interact with helper T cells both in tumors and in the tdLN and that checkpoint blockade drugs enhance these interactions. These findings show that a relatively small fraction of DCs is responsible

SUBMITTER: Chudnovskiy A 

PROVIDER: S-EPMC12419230 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature