An antibody targeting high-molecular-weight kininogen blocks contact system activation in a model of polymicrobial sepsis.

ABSTRACT:

Background

Polymicrobial sepsis is an infectious disease characterized by excessive inflammation and coagulation that is linked to more severe disease pathology, organ failure, and fatality. The plasma contact system is a protein cascade in the blood that can be activated by bacteria and contributes to both inflammation and coagulation.

Objectives

To determine if inhibiting the plasma contact system by targeting high-molecular-weight kininogen (HK) can exert a protective effect on bacteria-induced coagulation.

Methods

Polymicrobial cecal slurry (CS) was prepared from donor mice and used for ex vivo and in vivo experiments. CS was used in vivo to establish a murine model of polymicrobial sepsis. CS was incubated with mouse or human plasma ex vivo. Contact system activa

SUBMITTER: Badimon A 

PROVIDER: S-EPMC12422282 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature