Smarce1 fine-tunes cardiomyocyte proliferation in the embryonic zebrafish heart.

ABSTRACT:

Introduction

The molecular mechanisms regulating cardiomyocyte (CM) proliferation during heart development are essential for understanding regenerative processes but remain incompletely defined. While adult mammalian CMs are post-mitotic, zebrafish retain proliferative capacity throughout life. We aimed to identify genetic regulators that fine-tune CM proliferation during cardiac development.

Methods

Using an N-ethyl-N-nitrosourea (ENU) mutagenesis screen in zebrafish, we identified the embryonic-lethal mutant heart of stone (hos), which exhibits cardiac hyperplasia. Genetic mapping revealed a point mutation in smarce1, a component of the SWI/SNF chromatin remodeling complex. We performed morpholino knockdown, mRNA rescue, and Tet-On-driven myocardium-specific

SUBMITTER: Park DD 

PROVIDER: S-EPMC12426013 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature