Neonatal microglia replacement in mice modulates seizure severity in adulthood.

ABSTRACT: Microglia replacement therapy, where endogenous brain macrophages are depleted and replaced by adoptively transferred surrogates, holds promise for treating pediatric neurologic diseases, but little is known about how early-life microglia replacement impacts the brain. We sought to investigate how early postnatal microglia depletion and adoptive macrophage transfer, essential components of microglia replacement, durably impact neural circuits in a mouse model. Using both pharmacologic and genetic models, postnatal microglia depletion worsened adult seizure severity, mortality, and neuropathology in a chemical seizure model. Replacement of endogenous microglia by adoptive transfer of monocytes rescued this effect, while transfer of authentic microglia from a donor mouse did not, and even wo

SUBMITTER: O'Brien CA 

PROVIDER: S-EPMC12444772 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature