Adaptively evolved <i>Methylorubrum extorquens</i> with enhanced formate tolerance and its application in 3-hydroxypropionic acid production.

ABSTRACT: Methylorubrum extorquens AM1, a native formate-utilizing bacterium, has exhibited limited capacity to tolerate formate. In this study, we employed an adaptive laboratory evolution (ALE) strategy to develop an evolved strain FT3 derived from M. extorquens AM1, with enhanced formate tolerance. When cultivated with a mixture of carbon sources containing 90 mM formate and 30 mM methanol, the FT3 strain exhibited 5.3 times higher optical density (OD₆₀₀) compared to the parental strain. FT3 strain was shown to efficiently utilize both methanol and formate in experiments using ¹³C-labeled carbon sources. Furthermore, the mechanism underlying the enhanced formate tolerance in FT3 strain was investigated through a combination of DNA re-sequencing, transcriptome a

SUBMITTER: Mo X 

PROVIDER: S-EPMC12445348 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature