Library-Free Multiplexed Targeted Proteomics Enables Quantitative Protein-Level Genotyping.

ABSTRACT: Existing targeted mass spectrometry methods require synthetic standard peptides, manual scan scheduling, or data libraries to quantify target proteins. Here we present GoDig-LiF, which uses spectra and retention times predicted by the Prosit-TMT model in place of data libraries, enabling targeted proteomics with only a tandem mass tag-labeled sample, target list, and mass spectrometer. We applied GoDig-LiF to the quantification of mutated proteins in cancer cell lines, including KRAS G13D.

SUBMITTER: Shuken SR 

PROVIDER: S-EPMC12458936 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature