Proteomic profiling identifies miR-423-5p as a modulator of oncogenic metabolism in HCC.

ABSTRACT:

Background

Hepatocellular carcinoma (HCC) remains a significant clinical challenge due to limited diagnostic and therapeutic options. Non-coding RNAs (ncRNAs), such as microRNAs (miRNAs), play key roles in cancer biology. Our previous findings showed that miR-423-5p enhances anti-cancer effects on HCC patients treated with sorafenib by promoting autophagy. Here, we investigated the molecular mechanisms underlying miR-423-5p function through a comprehensive proteomic approach.

Methods

We generated an HCC cell line stably overexpressing miR-423-5p via lentiviral transduction. Total proteins were extracted from SNU-387 cells, enzymatically digested into peptides, and subsequently analysed by liquid chromatography-tandem mass spectrometry (LC-MS/M). Raw spectral data were proces

SUBMITTER: Luce A 

PROVIDER: S-EPMC12462032 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature