Altered carnitine-acylcarnitine profiles in levothyroxine-treated congenital hypothyroid patients with fatigue: An LC-MS/MS-based study from Bangladesh.

ABSTRACT: Congenital hypothyroidism (CH), characterized by insufficient thyroid hormone production at birth, is frequently associated with fatigue, particularly in cases with delayed diagnosis. This study employed liquid chromatography-tandem mass spectrometry (LC-MS/MS) to profile carnitine and acylcarnitines in late-diagnosed congenital hypothyroid patients receiving levothyroxine (LT4) therapy, with the aim of identifying metabolic alterations that may be associated with fatigue symptoms. A total of 56 late-diagnosed congenital hypothyroid patients and 107 age-, sex-, and BMI-matched healthy controls were enrolled. Blood samples were collected in EDTA tubes and as dried blood spots (DBS) on Whatman® 903 filter paper. LC-MS/MS was used to quantify free carnitine and 28 acylcarnitines, and plasma t

SUBMITTER: Begum MN 

PROVIDER: S-EPMC12463203 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature