Cataloging the potential functional diversity of Cacna1e splice variants using long-read sequencing.

ABSTRACT:

Background

The degree to which alternative RNA splicing influences the function and structure of voltage gated calcium channel (VGCC) splice variants is poorly understood. Here we used long-read RNA-sequencing to catalog rat Cacna1e (Cav2.3) splice variants, and computationally prioritize which are likely to impact channel function.

Result

We sequenced Cacna1e transcripts from rat thalamus using Oxford Nanopore sequencing yielding the structure of 2,110 Cacna1e splice variants. Of these, up to 154 had the potential encode for a functional channel based on predicted amino acid sequences. Our analysis revealed a total of 31 cassette splicing events (in various combinations) potentially affecting channel function, with three cassette exons appreciably expressed and conserved.

SUBMITTER: Bhuiyan SA 

PROVIDER: S-EPMC12465193 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature