Immunomodulatory Effects of <i>Lactobacillus brevis</i> NES-428 in a Hyperthyroidism Mouse Model: Potential Applications for Graves' Disease.

ABSTRACT: Background: Safe, microbiome-based interventions for autoimmune hyperthyroidism are lacking. We isolated the lactic acid bacterium NES-428 from kimchi and previously demonstrated that it shares 99% 16S-rRNA identity with Lactobacillus brevis reference strains, confirming NES-428 as a novel strain. Here we evaluated its immunomodulatory and anti-thyroid activity in cellular and murine models. Methods: Jurkat T cells (5 × 10⁶) were incubated with heat-killed NES-428 for 24 h and subsequently stimulated with phorbol 12-myristate 13-acetate/ionomycin (50 ng mL^-1/1 µg mL^-1) for 6 h; cytokine transcripts were quantified by qRT-PCR. Hyperthyroidism was induced in female BALB/c mice by three intramuscular injections of adenovirus-encoding human

SUBMITTER: Lee MG 

PROVIDER: S-EPMC12473064 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature