Establishment of an Advanced In Vitro Model for Pseudoexfoliation Syndrome and Glaucoma.

ABSTRACT:

Purpose

Pseudoexfoliation (PEX) glaucoma is caused by progressive accumulation of abnormal fibrillar aggregates in aqueous humor outflow tissues. The current lack of effective models for PEX research, replicating the key feature of PEX material production, represents a critical gap in understanding the molecular pathomechanisms and identifying specific therapeutic targets. We have developed an advanced in vitro model using stromal fibroblasts from iridectomy specimens of PEX glaucoma patients with an appropriate genetic background.

Methods

Peripheral iridectomy specimens were obtained from patients with PEX glaucoma (n = 35) during routine trabeculectomy. Stromal fibroblasts were enriched from heterogenous cell cultures. Extracellular matrix formation was stimulated and modu

SUBMITTER: Pulasani S 

PROVIDER: S-EPMC12476155 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature