Single-cell transcriptomic analyses provide insights into SPP1⁺ TAM-mediated immune suppression and CD8⁺ T cell dysfunction in lung cancer.

ABSTRACT:

Background

Metastatic lung carcinoma poses considerable treatment difficulties. It exhibits cellular characteristics that differ from those of early-stage cancer. Immunotherapy demonstrates enhanced hope for patients with advanced and metastatic lung cancer. Tumor-associated macrophages (TAMs) may be a contributing factor that diminishes the effectiveness of immunotherapy. Secreted phosphoprotein 1 (SPP1)⁺TAMs are considered to possess immunosuppressive characteristics, as their interaction with CD8⁺ T lymphocytes results in the exhaustion of these cells.

Methods

We analyzed single-cell RNA sequencing datasets of lung cancer metastases in order to examine the development of brain metastatic tumors. Furthermore, the cell-cell interactions between SPP1

SUBMITTER: Almgrami RT 

PROVIDER: S-EPMC12480184 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature