Impact of hcp and vgrG on Acinetobacter baumannii biofilm formation during infection of human pulmonary alveolar epithelial cells.

ABSTRACT: The purpose of this research is to examine the impact of key type VI secretion system (T6SS) proteins hemolysin coregulated protein (Hcp) and valine-glycine repeat protein G (VgrG) on the metabolism of Acinetobacter baumannii (A. baumannii). Homologous recombination technology was used to construct hcp knockout strain (ATCC17978Δhcp), vgrG knockout strain (ATCC17978ΔvgrG), and a combined hcp and vgrG knockout strain (ATCC17978ΔhcpΔvgrG), with the wild-type A. baumannii strain (ATCC17978) used as a control. These strains were co-cultured with human pulmonary alveolar epithelial cells (HPAEpiC), respectively. Subsequently, a non-targeted metabolomic analysis of the co-culture supernatant, bacteria, and cells was conducted using liquid chromatography-tandem mass spectrometry (LC-MS/MS). In th

SUBMITTER: Huang M 

PROVIDER: S-EPMC12494896 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature