Ribosomal protein L27 contributes to virulence and maduramicin resistance in Eimeria tenella.

ABSTRACT: Coccidiosis, which is primarily caused by Eimeria spp., poses a persistent challenge to poultry health and production worldwide. The emergence and spread of drug-resistant strains have significantly compromised the efficacy of anticoccidial therapies. We previously used RNA-seq to demonstrate that ribosomal protein L27 is differentially expressed in drug-sensitive and maduramicin-resistant strains of E. tenella (EtRPL27). In the present study, an RT-qPCR analysis showed that its expression is stage-specific, with the highest levels in sporozoites and second-generation merozoites. Immunofluorescence revealed both the cytoplasmic and partial surface localization of EtRPL27. Notably, EtRPL27 transcript levels were significantly elevated in a maduramicin-resistant strain relative to the drug-s

SUBMITTER: Yu Y 

PROVIDER: S-EPMC12509730 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature