A novel <i>C. elegans</i> respirometry assay using low-cost optical oxygen sensors.

ABSTRACT: Measurement of the oxygen consumption rate, or respirometry, is a powerful and comprehensive method for assessing mitochondrial function both in vitro and in vivo. Respirometry at the whole-organism level has been repeatedly performed in the model organism Caenorhabditis elegans, typically using high-throughput microplate-based systems over traditional Clark-type respirometers. However, these systems are highly specialized, costly to purchase and operate, and inaccessible to many researchers. Here, we develop a respirometry assay using low-cost commercially available optical oxygen sensors (PreSens OxoPlates^®) and fluorescence plate readers (the BMG FLUOstar), as an alternative to more costly standard respirometry systems. This assay uses standard BMG FLUOst

SUBMITTER: Dennis N 

PROVIDER: S-EPMC12557035 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature