Co-Occurrence and Molecular Characterization of ESBL-Producing and Colistin-Resistant <i>Escherichia coli</i> Isolates from Retail Raw Meat.

ABSTRACT: Background: The emergence of extended-spectrum β-lactamase (ESBL) producing and colistin-resistant Escherichia coli in retail meat poses a significant public health risk. Method: A total of 180 retail meat samples (chicken parts, internals, processed products; lamb; beef; fish) were purchased from markets and butcher shops across Turkiye. Presumptive ESBL-producing isolates were screened on chromogenic agar and phenotypically confirmed. Species identity was verified by uspA PCR, and resistance genes (blaCTX-M, blaTEM, blaOXA, blaSHV, mcr-1, mcr-2, mcr-3) were analyzed. Colistin MICs were determined by broth microdilution, while antimicrobial susceptibility of ESBL-positive isolates was assessed by disk diffusion.

SUBMITTER: Telli AE 

PROVIDER: S-EPMC12564342 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature