A luminescence-based point-of-care HIV viral load test for antiretroviral therapy monitoring.

ABSTRACT: Effective monitoring of HIV treatment remains constrained by limited access to affordable, sensitive, and user-friendly viral load diagnostics, particularly in resource-limited settings. To address this critical gap, a fully automated, point-of-care device was developed that integrates microfluidic sample processing with ultrasensitive bioluminescence-based viral load detection. Upon simple sample loading, the system automates all steps, capturing multiple HIV subtypes with antibody-coated magnetic beads without fold-change, amplifying bioluminescence via enzyme cascades, and quantifying viral load using a low-cost optical sensor. Analytical validation using 87 HIV-spiked plasma samples confirmed a 95 copies per mL detection limit and reliable quantification of viral loads. Clinical

SUBMITTER: Kim S 

PROVIDER: S-EPMC12574115 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature