Synthetic Polyploidisation Enhances Fusarium graminearum Tolerance in Wheat by Reshaping the Transcriptome and Strengthening the Microbiome.

ABSTRACT: Polyploidisation is a natural evolutionary mechanism that enhances plant stress tolerance and environmental adaptability; however, its impact on microbiome homeostasis remains poorly understood. In this study, we selected a nascent euploid synthetic hexaploid wheat line (HG116; 2n = 6x = 42, BBAADD) by selfing a triploid F1 hybrid of Triticum turgidum L. ssp. durum (Langdon, LDN; 2n = 4x = 28, BBAA) and Aegilops tauschii Coss. (SY41; 2n = 2x = 14, DD). We investigated the effects of synthetic polyploidisation on gene expression in roots, the root-associated microbiome and tolerance to Fusarium graminearum. Transcriptomic analysis revealed that polyploidisation in HG116 predominantly upregulated genes, which were enriched in stress- and defence-related pathways, particularly those involved

SUBMITTER: He X 

PROVIDER: S-EPMC12576463 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature