Optimized Kappa-Index Test Protocol to Detect Intrathecal Immunoglobulin Synthesis in Multiple Sclerosis.

ABSTRACT:

Background

Kappa free light chain (KFLC)-based Kappa-index (K-index) can substitute oligoclonal banding (OCB) to detect intrathecal immunoglobulin synthesis. Cutoffs for positive results vary; therefore, further comparison of different approaches is necessary to optimize K-index for diagnostic utilization.

Methods

We analyzed 432 paired CSF/serum samples and selected 77 OCB-positive patients with multiple sclerosis (MS), clinically isolated syndrome (CIS) that did not progress to MS in > 10 years, and other neuroinflammatory diseases (non-MS). K-index subtests were measured on the Optilite platform (The Binding Site) and OCB with isoelectric focusing on the Hydrasys platform (Sebia).

Results

OCB positive cases had a higher K-index than OCB negative. The MS group had a

SUBMITTER: Bjorklund J 

PROVIDER: S-EPMC12581111 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature