NDC80 promotes epithelial to mesenchymal transition of esophageal squamous cell carcinoma through macrophages polarization and PI3K/AKT pathway activation.

ABSTRACT:

Objective

Esophageal squamous cell carcinoma (ESCC) is a lethal malignancy with limited therapeutic options, primarily due to its aggressive metastatic behavior. This study aimed to elucidate the molecular drivers of ESCC metastasis by identifying a critical oncogene and investigate its functional mechanisms.

Methods

Using integrated bioinformatics screening, we identified NDC80 as a potential regulator of metastasis. The role and underlying mechanisms of NDC80 in ESCC progression have remained to be fully elucidated. We conducted in vitro functional assays-including real-time PCR, western blotting, and flow cytometry-to assess the effects of NDC80 on epithelial-mesenchymal transition (EMT) and malignant behavior. Mechanistic studies such as co-culture systems and ELISA, wer

SUBMITTER: Shu J 

PROVIDER: S-EPMC12599082 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature