CRATER tumor niches facilitate CD8⁺ T cell engagement and correspond with immunotherapy success.

ABSTRACT: T cell-mediated tumor killing underlies immunotherapy success. Here, we used long-term in vivo imaging and high-resolution spatial transcriptomics of zebrafish endogenous melanoma, as well as multiplex imaging of human melanoma, to identify domains facilitating the immune response during immunotherapy. We identified cancer regions of antigen presentation and T cell engagement and retention (CRATERs) as pockets at the stroma-melanocyte boundaries of zebrafish and human melanoma. CRATERs are rich in antigen-recognition molecules, harboring the highest density of CD8⁺ T cells in tumors. In zebrafish, CD8⁺ T cells formed prolonged interactions with melanoma cells within CRATERs, characteristic of antigen recognition. Following immunostimulatory treatment, CRATERs expanded

SUBMITTER: Ludin A 

PROVIDER: S-EPMC12604482 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature