Prm-PASEF-Based Quantification and Isomeric Model for Extended Coverage of Human Plasma Lipidome in Parkinson's Disease.

ABSTRACT: This study introduces a clinical lipidomics platform leveraging fragment-based quantification on parallel reaction monitoring (PRM)-parallel accumulation serial fragmentation (PASEF) for lipid quantification. An isomeric model, termed "SN regression model", built on specific PASEF-fragment ion patterns, was developed for the quantification of coeluting sn positional isomers without prior derivatization. This PASEF-isomeric lipidomics aids in the resolution and quantification of 176 lipid isomers coeluting in chromatography and/or ion mobility dimensions, expanding the lipidome quantitative coverage to 481 plasma lipids covering 14 lipid subclasses with CV <40% for 32 plasma replicates. We demonstrated the method's advantage for clinical research by detailed quantitative lipidomic phenotypi

SUBMITTER: Baker D 

PROVIDER: S-EPMC12613151 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature