<i>CTSB</i> mediates oxidative stress and intestinal epithelial barrier disruption in intestinal ischemia‑reperfusion injury.

ABSTRACT: Intestinal ischemia‑reperfusion (I/R) injury is a clinical condition that leads to severe intestinal damage, inflammation and oxidative stress. While cathepsin B (CTSB) has been implicated in these pathophysiological processes, its precise role in mediating I/R‑induced injury remains poorly understood. The present study aimed to elucidate how CTSB knockdown influences oxidative stress, inflammatory responses and the integrity of the intestinal epithelial barrier in intestinal epithelial Caco‑2 cells subjected to I/R injury. To identify key genes implicated in I/R injury, a comprehensive analysis was conducted using differential expression profiling and protein‑protein interaction network analysis of the GSE37013 dataset. To simulate I/R damage in vitro, an oxygen‑gluco

SUBMITTER: He S 

PROVIDER: S-EPMC12635601 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature