The Translational Coupling of Daidzein Reductase and Dihydrodaidzein Racemase Genes Improves the Production of Equol and Its Analogous Derivatives in Engineered Lactic Acid Bacteria.

ABSTRACT: Equol (EQ) and its analogous derivatives 5-hydroxy-equol (5-OH-EQ) and 5-hydroxy-dehydroequol (5-OH-D-EQ) are isoflavones which benefit human health. They are produced from daidzein and genistein, respectively, in the gut by microorganisms harboring the genes daidzein reductase (dzr), dihydrodaidzein racemase (ifcA), dihydrodaidzein reductase (ddr) and tetrahydrodaidzein reductase (tdr). Since the production of these isoflavones is of interest due to their great-health benefits for humans, the heterologous expression of dzr, ddr, tdr and ifcA from Slackia isoflavoniconvertenes DSM 22006T in lactic acid bacteria (LAB) was used as a strategy to produce EQ, 5-OH-EQ and 5-OH-D-EQ in soy beverages. However, efficient production of t

SUBMITTER: Langa S 

PROVIDER: S-EPMC12645574 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature