Enhancing Stallion Semen Cryopreservation: Selected Antioxidant Extracts and Sperm Freezability.

ABSTRACT: Cryopreservation of equine semen remains challenging due to pronounced individual variability in cryotolerance. Because freezing induces oxidative stress and spermatozoa are particularly susceptible to such damage, this study aimed to comparatively evaluate the effects of natural extracts from nutraceutical compounds with high antioxidant activity, specifically matcha, spirulina, and horseradish, as well as quercetin, a well-known antioxidant molecule. These compounds were added to the freezing extender, and semen from 12 Salernitano stallions (48 ejaculates in total) was analyzed. Several parameters were assessed, including sperm kinetics, bioenergetics, oxidative and nitrosative stress markers, and the sperm DNA fragmentation index, both before and after cryopreservation. Neither the nat

SUBMITTER: Boni R 

PROVIDER: S-EPMC12649543 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature