Validation of <i>GDAP1</i> and <i>HECW2</i> as Epigenetic Markers of Alcohol Use Disorder in Blood and Brain.

ABSTRACT: Alcohol use disorder (AUD) is associated with widespread epigenetic alterations, including changes in DNA methylation (DNAm). This multi-cohort study validated and extended previous findings on DNAm of HECW2 and GDAP1 in AUD, assessed sex differences, and explored DNAm in blood and brain tissue in humans and rats. DNAm was measured via pyrosequencing in human blood (N_Ctrl = 341, N_AUD = 258), postmortem frontal cortex (Brodmann area 9; discovery cohort: N_Ctrl = 10, N_AUD = 13, replication cohort: N_Ctrl = 64, N_AUD = 55) and rat blood and medial prefrontal cortex (N_Ctrl = 16, N_AUD = 15). Gene expression was assessed in human postmortem brain by quantitative real-time PCR. AUD-associated DNAm d

SUBMITTER: Wiegand A 

PROVIDER: S-EPMC12652996 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature