Leveraging Transcriptome Insights and <i>GsHZ4</i> Gene Expression to Improve Alkaline Tolerance in <i>Lupinus angustifolius</i>.

ABSTRACT: Soil alkalinity severely restricts the cultivation of Lupinus angustifolius, a valuable legume. Wild soybean (Glycine soja) is a leguminous plant with extremely strong alkaline resistance (pH 8.5). Transferring the alkali-tolerant genes from wild soybeans into lupinus can effectively enhance the alkali tolerance. In this study, we combined transcriptome profiling and genetic transformation to elucidate the molecular basis of alkaline stress response in lupinus. RNA-seq analysis of root tips under acid (HCl, pH 4.0) and alkali (NaHCO₃, pH 8.5) stress revealed 104,353 annotated unigenes, with differential expression patterns highlighting enrichment in cellular component, binding, and catalytic activity categories. KEGG pathway analysis indicated that early responses

SUBMITTER: Tang J 

PROVIDER: S-EPMC12656323 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature