Rational Design of a Potent Two-Phage Cocktail Against a Contemporary <i>Acinetobacter baumannii</i> Strain Recovered from a Burned Patient at the Lausanne University Hospital.

ABSTRACT: Acinetobacter baumannii is a critical public health threat, particularly with the rise in multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains that limit treatment options. Phage therapy, which uses bacteriophages to target bacteria, offers a promising alternative. We isolated an XDR strain (Ab125) from a burn wound infection and screened 34 phages, identifying vB_AbaM_3098 as the only effective candidate. However, resistance rapidly emerged, producing a derivative strain (Ab139). Interestingly, Ab139, though resistant to vB_AbaM_3098, became susceptible to six previously inactive phages. While various potential determinants were identified through comparative genomics and proteomics, the mechanism causing phage resistance to vB_AbaM_3098 and simultaneous susceptib

SUBMITTER: de Villiers de la Noue H 

PROVIDER: S-EPMC12656882 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature