Manganese oxide (MnO_x) as peroxidase-mimicking nanozymes with valence-dependent activity for single-use colorimetric bioassays.

ABSTRACT: Manganese oxide (MnO_x)-based nanozymes have attracted growing interest due to their low cost, high catalytic robustness, and adjustable oxidation states that influence enzyme-mimicking performance. In this study, we report a systematic approach to synthesize MnO_x nanocomposites with tunable Mn valence states via a two-step pyrolysis of Mn-BTC precursors, enabling us to explore their valence-dependent peroxidase-like activities. Among the synthesized forms, Mn₃O₄ exhibited the highest catalytic efficiency by facilitating the decomposition of H₂O₂ to generate hydroxyl radicals (•OH), which oxidize tetramethylbenzidine (TMB) to produce a blue-colored product. The catalytic mechanism was supported by UV-vis spectroscopy and fluorescence assays. Kinet

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC12658014 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature