Immune landscape in liver of neonatal mice with phlebotomy-induced anemia.

ABSTRACT:

Background

Severe anemia is a common comorbidity in preterm infants in the neonatal intensive care unit, which is caused by phlebotomy, low erythropoietin levels, low red blood cell (RBC) lifespan, and exacerbated by the underlying erythropoietic immaturity. Anemia causes tissue hypoxia, which may alter the hematopoiesis niche in the liver. This study utilized our preclinical mouse model of phlebotomy-induced anemia (PIA) to investigate the immune cell atlas in the liver.

Methods

C57BL/6 mice were subjected to timed phlebotomy between postnatal days 2-10 to induce severe anemia. Immune cells in anemic liver were characterized by Single-cell (sc) RNA-sequencing and a flow cytometry approach.

Results

The scRNA-seq analysis revealed that PIA is associated with an altered

SUBMITTER: Ramatchandirin B 

PROVIDER: S-EPMC12659965 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature