Engineering ATP Import in Yeast Uncovers a Synthetic Route to Extend Cellular Lifespan.

ABSTRACT: Aging results from the gradual accumulation of molecular damage as a result of cellular processes and is characterized by impaired functions, most notably an age-related decline in ATP production. However, the causal relationship between cellular ATP homeostasis and aging has not been established. In this study, we used a novel approach by harnessing a nucleotide transporter from a eukaryotic intracellular parasite to facilitate the direct import of extracellular ATP into budding yeast cells, enabling us to effectively manipulate their intracellular ATP levels. We found that depletion of ATP significantly reduces lifespan, while the supplementation of ATP in the growth medium fully restores it thereby extending lifespan. Moreover, gene expression analysis revealed that elevated ATP levels

SUBMITTER: Oz N 

PROVIDER: S-EPMC12667902 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature