Protection against H9N2 challenge in chickens via <i>Salmonella</i> with a <i>focA</i> mutation delivered by a dendritic cell-targeted avian influenza virus NA protein tetramer.

ABSTRACT: Vaccination is considered one of the most effective methods for preventing avian influenza. The influenza neuraminidase (NA) enzyme is a promising candidate for a conserved influenza vaccine, offering long-term and effective cross-protection against the disease. The aim of this study is to develop a Salmonella-vectored vaccine with strong protective efficacy against H9N2 avian influenza virus. We utilized the antibiotic-free balanced-lethal system of regulated delayed-lysis Salmonella (χ11218) as the starting strain to construct a focA gene-deleted Salmonella (χYL57) strain. To optimize the vaccine design, we fused a tetravalent NA protein (tetramer-NA) with a chicken dendritic cell-targeting peptide (chDCpep) and delivered it via χ11218 or χYL57, yielding the c

SUBMITTER: Ge C 

PROVIDER: S-EPMC12671097 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature