High-fructose diet induces depressive-like behaviors and short-term memory deficits through hippocampal neurogenesis impairment via neural stem cell dysfunction.

ABSTRACT:

Background

Neural stem cells (NSCs), crucial for brain function and repair, are disrupted by high-fructose diet (HFrD) in proliferation and survival, linking to neurogenesis impairment and neuropsychiatric risks. Mechanistic insights remain undefined.

Methods

Comprehensive behavioral assessments were conducted on HFrD mice, including the tail suspension test (TST) and sucrose preference test (SPT) for depressive-like behaviors, elevated plus maze (EPM) and open field test (OFT) for anxiety-like behaviors, as well as novel object recognition (NOR) and Morris water maze (MWM) for cognition. Hippocampal NSCs and newborn neurons were quantified by immunofluorescence, and fructose-treated NE-4C cells underwent RNA sequencing (RNA-seq) analysis coupled with measurements of prolife

SUBMITTER: Wang Q 

PROVIDER: S-EPMC12673773 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature