Biosynthesis of H₂S and Siderophores Targeting Gram-Negative Bacterial Resistance to Reactive Oxygen Species.

ABSTRACT: Reactive oxygen species (ROS) are a promising alternative bactericide. However, it is questioned that bacteria can potentially develop resistance to ROS, similar to their resistance against antibiotics and silver. Herein, it is reported that Gram-negative bacteria, including Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumoniae, develop resistance to ROS after six repeated exposures. Notably, ROS minimum inhibitory concentration of Pseudomonas aeruginosa significantly increases to 256-fold after ten passages. The resistance mechanism predominantly originates from the intensified biosynthesis of the highly reductive hydrogen sulfide (H₂S) and pyoverdine (PVD) siderophores, effectively neutralizing ROS. Simultaneously, PVD transports Fe³⁺ from the extracel

SUBMITTER: Mao C 

PROVIDER: S-EPMC12677624 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature