Functional S-persulfidation exploration reveals a novel regulatory mechanism of HNF1α.

ABSTRACT: Protein S-persulfidation, a post-translational modification mediated by hydrogen sulfide (H₂S), plays an essential role in regulating protein function. However, current profiling methods, mainly based on liquid chromatography-tandem mass spectrometry (LC-MS/MS), tend to detect abundant proteins and often miss low-abundance but important regulators like transcription factors (TFs). To better understand the S-persulfidation landscape, we integrated datasets from three species-Homo sapiens, Mus musculus, and Arabidopsis thaliana-and found that TFs are strikingly underrepresented. To address this gap, we applied oxidative proteomics data from the Oximouse database, which reflects age-related redox changes, and identified hepatocyte nuclear factor 1α (HNF1α) as a candidate S-persulfi

SUBMITTER: Huang Y 

PROVIDER: S-EPMC12681906 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature