Characterization of a hypersporulating strain derivative <i>of Clostridioides difficile</i> R20291.

ABSTRACT: Clostridioides difficile is a Gram-positive and obligate anaerobic spore-former pathogen. C. difficile spores are essential for the transmission and recurrence of C. difficile infections (CDI). A major challenge in sporulation studies in C. difficile is the low yield and asynchronous nature of this process. In this work, a hypersporulating strain, derivative of R20291, with an early sporulation onset and enhanced sporulation efficiency was isolated by serendipity. This strain had 1000-fold higher sporulation efficiency than the parental R20291 strain in sessile culture conditions. Electron micrographs revealed that spores of both strains have similar hair-like projections, electron-dense outer exosporium layer features. Whole genome sequencing and genomic analys

SUBMITTER: Cid-Rojas F 

PROVIDER: S-EPMC12687779 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature