Age-Related Changes in Neuron-Microglia Interaction Mediated by Fractalkine Under Inflammatory Conditions.

ABSTRACT: Ageing results in an increased microglial activation and neuroinflammation. We are interested in assessing ageing-dependent changes in the amount and fractalkine (CX3CL1) proteoforms participating in neuron-microglia crosstalk that could be involved in microglia activation. We analysed age-dependent changes in CX3CL1, CX3CR1, and TGFβ mRNAs using RT-qPCR and CX3CL1 proteoforms using Western blot, in 3 to 20-month-old WT mice and an inflammatory mouse model (SRA^-/-) treated with 0.5 mg/kg of intraperitoneal LPS, 2 ng of intrathecal TGFβ, or a vehicle. CX3CL1, CX3CR1, and TGFβ were affected by ageing. CX3CL1 mRNA was similar in young and adult mice but decreased by 52% in >20-month-old mice; adult mice showed a 3-fold increase in 70 kDa soluble CX3CL1. CX3CR1 showed a progressive

SUBMITTER: von Bernhardi R 

PROVIDER: S-EPMC12692065 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature