Genome-wide Identification of conditionally essential genes supporting <i>Streptococcus suis</i> growth in serum and cerebrospinal fluid.

ABSTRACT: Streptococcus suis is a major cause of sepsis and meningitis in pigs, and zoonosis through the emergence of disease-associated lineages. The ability of S. suis to adapt and survive in host environments, such as blood and cerebrospinal fluid (CSF), is important for pathogenesis. Here, we used Transposon Sequencing (Tn-seq) coupled with Nanopore sequencing to identify conditionally essential genes (CEGs) for the growth of S. suis P1/7 in active porcine serum (APS) and CSF derived from choroid plexus organoids. To our knowledge, this is the first successful application of ONT to Tn-library screening, enabling rapid local runs and a publicly available analysis pipeline. Through comparative fitness analyses, we identified 33 CEGs that support growth in APS and 25 CEGs in CS

SUBMITTER: Juanpere-Borras M 

PROVIDER: S-EPMC12707516 | biostudies-literature | 2026 Dec

REPOSITORIES: biostudies-literature