Hemolysis-induced hepatic ferroptosis following xenotransfusion of genetically modified pig red blood cells.

ABSTRACT: To overcome cross-species immunological barriers, researchers utilize gene editing to remove the expression of three major carbohydrate xenoantigens (α-Gal, Neu5Gc, and Sd(a)) and insert genes, such as human protective genes (hCD55 and hCD39). This study aimed to investigate the mechanisms underlying long-term hepatic injury following xenotransfusion of genetically modified pig red blood cells (pRBCs). We xenotransfused pRBCs genetically engineered with GGTA1^-/-(α-Gal ^-); CMAH^-/-(Neu5Gc^-); β4GALNT2^-/-(Sd(a) ^-); hCD55; hCD39 into a non-human primate (NHP) model of acute hemorrhage hemolysis and evaluated the long-term immune response to xenogeneic RBCs in the liver, a key organ for RBC metabolism. Immediately after xenotransf

SUBMITTER: Shin HY 

PROVIDER: S-EPMC12711946 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature