BDH1 acetylation at K116 modulates milk fat production in dairy goats.

ABSTRACT:

Background

Goat milk is increasingly recognized for high digestibility and a distinctive compositional profile. Protein acetylation, an important post-translational modification, regulates biosynthetic and metabolic pathways. This study aimed to identify critical acetylated proteins and specific modification sites involved in milk production and component synthesis in dairy goats, thereby elucidating the molecular mechanisms of lactation. We performed a comparative TMT-based acetylomic and proteomic analysis of mammary tissues from Saanen dairy goats during peak lactation and the dry period using LC-MS/MS. A candidate acetylation site was further investigated in goat mammary epithelial cells (GMECs) through site-directed mutagenesis and lipid metabolic assays, establishing function

SUBMITTER: Xiong T 

PROVIDER: S-EPMC12720437 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature