Sequential Inflammatory and Matrisome Programs Drive Remodeling of the Mouse Carotid-Jugular Arteriovenous Fistula.

ABSTRACT: The mouse carotid-jugular arteriovenous fistula (AVF) is a widely adopted surgical model to study venous remodeling after AVF creation. Despite its increasing use, the extent to which this model recapitulates the cellular and molecular remodeling processes observed in humans remains uncertain, which is essential for validating its translational relevance. Using bulk and single-cell RNA sequencing, we have depicted the transcriptional and cellular evolution of the mouse jugular vein after AVF anastomosis. Global transcriptomic profiling revealed that venous remodeling begins with a robust inflammatory response, followed by a prominent extracellular matrix (ECM) remodeling phase that peaks at postoperative day 10. Single-cell analyses confirmed the role of macrophage (3-fold) and neutrophil

SUBMITTER: Stoyell-Conti FF 

PROVIDER: S-EPMC12731578 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature