Identification and characterization of GHR gene promoter of yak.

ABSTRACT: Animal growth and development are regulated by the growth hormone receptor (GHR). To elucidate the transcriptional regulation mechanism of the yak GHR gene, we cloned its proximal promoter region and performed bioinformatic analyses. Deletion constructs were generated to produce fragments of the proximal promoter, and the fragment exhibiting the highest activity was identified using a dual-luciferase reporter assay system. Moreover, the functionality of the transcription factor binding sites was confirmed via site-directed mutagenesis and electrophoretic mobility shift assay (EMSA). Bioinformatics analysis revealed two transcriptional initiation sites were located in the upstream regulation region at positions - 277/ - 327 and - 1734/ - 1784 bp, and CpG islands were located at - 552/ - 654

SUBMITTER: Wu X 

PROVIDER: S-EPMC12764550 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature