Quality-by-design based RP-HPLC analytical method for the estimation of quercetin in silver-metal organic frameworks (AgMOF): a Box-Behnken optimization approach.

ABSTRACT: An efficient, accurate, sustainable, and reproducible chromatographic method for Quercetin estimation was developed, optimized, and further validated using a Design of Experiments approach in compliance with ICH guidelines. The Box-Behnken Quality-by-Design optimized critical parameters, yielding robust performance, excellent linearity (R² = 0.9996, 0.05-50 μg/mL), and high sensitivity (LOD: 40.27 ng/mL, LOQ: 122.03 ng/mL). Deliberate modifications evaluated by analysis of variance verified the method's significance (P < 0.0001). The method utilises a Phenomenex HyperClone C18 LC Column (250 × 4.6 mm; 5 μm, 130 Å) with an isocratic mobile phase consisting of acidified water (0.1% v/v Triethylamine, pH 3.0 ± 0.05) and Acetonitrile (55:45% v/v). Forced degradation studies revealed

SUBMITTER: Mukharya A 

PROVIDER: S-EPMC12764978 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature