Tropism, replication competence, and cellular responses of HAdV-14p1 in human airway organoids.

ABSTRACT: Human adenovirus type 14p1 (HAdV-14p1) is associated with severe respiratory illness. Using human airway organoids, we characterized the cellular tropism, infection dynamics, and cellular response to HAdV-14p1, and evaluated the antiviral efficacy of cidofovir. HAdV-14p1 robustly infected and replicated in human airway organoids, particularly targeting ciliated, basal, and goblet cells. CCL5, CXCL10, CXCL11, and TNFSF13B were significantly upregulated upon HAdV-14p1 infection, indicating inflammatory activation. Pathway enrichment analysis revealed that upregulated genes were enriched in p53 signaling, cytokine-cytokine receptor interaction, and NOD-like receptor signaling pathways, whereas downregulated genes were linked to focal adhesion, tight junction, metabolic pathways, and oxidative

SUBMITTER: Guo W 

PROVIDER: S-EPMC12765093 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature