Simultaneous and sensitive quantification of protein and low molecular weight persulfides, polysulfides and H₂S in biological samples.

ABSTRACT: H₂S reversibly modifies low molecular weight (L_MWSH) and protein (PrSH) thiols to form persulfides (RSS^-) and polysulfides (RS(S)_nS^-) for antioxidant defence and regulation of activity. However, our understanding of the biological significance of these processes is hampered by our inability to quantify these modifications. We develop a sensitive LC-MS/MS procedure that traps the sulfur atom of H₂S, and the terminal sulfur atom of RSS^- and RS(S)_nS^- as diagnostic products in biological samples. In parallel, we also trap internal S atoms of RS(S)_nS^-, enabling quantification of H₂S, RSS^- and RS(S)_nS^-. L_MWS(S)_n

SUBMITTER: Miljkovic JL 

PROVIDER: S-EPMC12769671 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature