Multi-proteomics reveals integrated metabolic and regulatory networks for xylan catabolism in <i>Streptomyces</i> sp. SirexAA-E.

ABSTRACT: An insect-associated bacterium, Streptomyces sp. SirexAA-E (SirexAA-E) secretes plant biomass-degrading enzymes depending on the available cell wall materials. SirexAA-E readily utilizes xylan, one of the major hemicelluloses. However, the enzyme composition and pathways supporting xylan metabolism in SirexAA-E and other Streptomycetes have not been reported. We aimed to understand changes in both extracellular and intracellular protein productions by cultivating SirexAA-E in defined media containing either xylose, xylobiose, or xylan. Proteomics showed each carbon source gave specific extracellular protein composition when compared to glucose. Furthermore, intracellular proteomics using a pair-wise Tandem Mass Tag (TMT)-labeling LC-MS/MS identified 1,037 proteins with changes in th

SUBMITTER: Nagano T 

PROVIDER: S-EPMC12772271 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature