Evaluation of Laboratory Techniques for the Diagnosis of Leptospira-Associated Equine Recurrent Uveitis (ERU) With Focus on the Goldmann-Witmer Coefficient.

ABSTRACT:

Purpose

To evaluate different laboratory procedures for determining the etiologic diagnosis of equine recurrent uveitis regarding intraocular infection with Leptospira spp. and to establish a diagnostic guideline.

Material and methods

Eighty horses with a history of ERU were ophthalmologically examined. Serum and aqueous humor were collected. Total protein, albumin level, and MAT against Leptospira spp. were evaluated on serum and aqueous humor. PCR for Leptospira spp., EHV-1 and -4 was performed on aqueous humor. Goldmann-Witmer coefficient (GWC) and C-value (CC) were calculated based on MAT. In 42 cases, an additional ELISA was initiated.

Results

Forty-six female and 34 male horses of different breeds (mean age 10.9 years; range 3 to 31) were included. By MAT 56/80

SUBMITTER: Kirmse L 

PROVIDER: S-EPMC12790138 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature