Exploring Metalloproteome Remodeling in Calprotectin-Stressed <i>Acinetobacter baumannii</i> Using Chemoproteomics.

ABSTRACT: The growth of bacterial pathogens is limited by nutritional immunity, where the infected host deploys transition metal scavenging proteins including calprotectin (CP) to starve the bacterium of essential transition metals. Prior work reveals that CP induces a significant Zn- and Fe-starvation response in the Gram-negative opportunistic pathogen Acinetobacter baumannii in liquid culture. Here, we employ a quantitative chemoproteomics platform to pinpoint changes in abundance-corrected cysteine reactivity─and by extension cellular metal occupancy in metalloenzymes─that occur when A. baumannii is challenged with physiological CP in liquid culture relative to an untreated WT control. Changes in protein abundance with CP stress reveal a pronounced Zn-limitation and Fe-starvation r

SUBMITTER: Osterberg MK 

PROVIDER: S-EPMC12797879 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature