Temporal transcriptomic profiling of bone autograft healing reveals dynamic immune, vascular, and osteogenic programs.

ABSTRACT: Bone autograft healing is a highly orchestrated process that integrates immune activation, vascular ingrowth, and osteogenic remodeling. To define the molecular and cellular programs driving early autograft integration, bulk and single-cell RNA sequencing was used to analyze graft-associated tissues over 14 days in a murine periosteal-mediated autograft model. Global transcriptomic analysis revealed rapid and dynamic remodeling, with maximal gene expression changes occurring within the first week. The first 48 h were dominated by pro-inflammatory signaling, including TNF, IL-1, TLR, and MAPK pathways, accompanied by transcriptional signatures of phagocytosis and cellular clearance. These early inflammatory programs gave way to pro-regenerative signals, including activation of HIF-1, PI3K-A

SUBMITTER: Mereness JA 

PROVIDER: S-EPMC12815593 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature