Regulation of indigoidine production in <i>Vogesella indigofera</i> by a novel two-component system.

ABSTRACT: Indigoidine is a blue pigment synthesized by several bacteria, including Vogesella indigofera. Industrial production of indigoidine has been a research focus, but less is known about why bacteria make this pigment or how its biosynthesis is regulated. We isolated V. indigofera strain OSW_575 and investigated the basis for its indigoidine production using genomic and genetic approaches. Mutation of the indigoidine synthase gene igiD eliminated pigment production, and complementation restored it. A transposon mutagenesis screen uncovered 34 mutations across 20 genes that affect pigment production, including some involved in metabolism, translation, protein homeostasis, and regulation. Three chaperones that combat misfolded proteins, dnaK, dnaJ, and grpE<

SUBMITTER: Babcock M 

PROVIDER: S-EPMC12826045 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature